I did a search on the forum and found out a bit, but it was not much. I usually keep my ants in plastic containers with the test tubes and water on the bottom and an oil layer up top to keep them from climbing out. I then place a lid with holes on it to keep the air flow going (and believe or not to keep the mice in the garage from eating them). What I have realized is that CO2 (carbon dioxide) is heavier than O2 (oxygen). Are my ants in danger of suffocation? How do you tell when they are out of oxygen? The ants I have are your typical back yard red ants.